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Autoimmune and Inflammation
自免与炎症疾病模型总览
自免疾病模型
实验性自身免疫性脑脊髓炎 (EAE)
MOG35-55/MOG1-125-induced EAE in mice
MBP-induced EAE in rats
PLP139-151-induced EAE in mice
类风湿性关节炎 (RA)
Collagen-induced arthritis (CIA) in mice and rats
Collagen antibody-induced arthritis (CAIA) in mice
Adjuvant-induced arthritis (AIA) in rats
系统性红斑狼疮 (SLE)
Pristane-induced lupus in mice
MRL/lpr mice lupus model
炎症性肠病 (IBD)
DSS-induced ulcerative colitis in mice
TNBS-induced crohn's disease in mice and rats
CD4+CD45RBhigh T cell-induced colitis in mice
过敏疾病模型
特应性皮炎 (AD)
Oxazolone-induced atopic dermatitis in mice
MC903-induced dermatitis in mice
DNCB-induced dermatitis in mice and rats
FITC-induced dermatitis in mice
银屑病 Psoriasis
Imiquimod-induced psoriasis in mice
IL-23-induced ear inflammation in mice and rats
哮喘 Asthma
OVA-induced asthma in mice
HDM-induced asthma in mice
迟发性超敏反应 (DTH)
Oxazolone-induced DTH in mice
KLH-induced DTH in mice
纤维化模型
肝纤维化 Liver Fibrosis
CCl4-induced liver fibrosis in mice and rats
DMN-induced liver fibrosis in mice and rats
TAA-induced liver fibrosis in mice and rats
Bile Duct Ligation (BDL) model in mice and rats
特发性肺纤维化 (IPF)
Bleomycin-induced pulmonary fibrosis in mice
肾纤维化 Kidney Fibrosis
Folic acid-induced kidney fibrosis in mice
短期炎症模型
LPS-induced Acute Inflammation Model
APAP-induced Acute Liver Injury Model
T cell Dependent Antibody Response (TDAR) Model
KLH-induced TDAR in mice
Eosinophilic Gastroenteritis (EG)
OVA-induced eosinophilic gastroenteritis in mice
Air Pouch Model
Carrageenan-induced air pouch in mice and rats
rhIL-13-induced air pouch in mice
移植物抗宿主病 (GVHD)
Graft-versus-Host Disease (GvHD)
GvHD induced by X-ray irradiation in mice
GvHD without irradiation in rats
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实验性自身免疫性脑脊髓炎 (EAE)Case Study: MOG35-55-induced EAE model实验动物: C57BL/6, 11 weeks, female造模试剂: MOG/CFA emulsion + PTX造模方法: Immunization: day 0, s.c., on back, PTX: day 0, day 1, i.p.结果输出临床观察: 临床评分, 体重组织病理学: H&E, LFB染色, IHC细胞, 分子水平检测: 流式分析FACS, 细胞因子分析(CBA, MSD, ELISA)临床和组织学评估
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类风湿性关节炎 (RA)Case Study: 牛II型胶原诱导的关节炎(CIA)模型实验动物: DBA/1, 7-8 weeks, male造模试剂: 牛II型胶原+ CFA造模方法: Immunization: day 0, i.d., tail; Booster: day 21结果输出临床观察: 临床评分, 体重组织病理学: H&E, IHC, IF分子水平检测: 细胞因子分析(CBA, MSD, ELISA)临床和组织学评估
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系统性红斑狼疮 (SLE)Case Study: Pristane-induced SLE Model实验动物: Balb/c, 6-8 weeks, female造模试剂: 姥鲛烷 Pristane造模方法: 0.5ml, i.p., once.结果输出临床观察: 体重组织病理学: H&E、IHC or IF尿生化检测: 肌酐Creatinine, 尿蛋白UTP, 微量白蛋白mALB, 尿素氮UREA细胞, 分子水平检测: Anti-dsDNA IgG, 流式分析FACS, 细胞因子分析(CBA, MSD, ELISA)血清Anti-dsDNA IgG分析
SummaryA significant increase of anti-dsDNA IgG can be observed in serum after pristane induction.Serum anti-dsDNA IgG levels exhibited a time-dependent elevation with prolonged induction duration.组织病理学分析
Pristane诱导16周，小鼠四肢出现关节肿胀现象，免疫荧光 (IF Staining)结果显示，IgG和C3在小鼠关节出现沉积；HE染色结果显示，小鼠关节滑膜增生、软骨破坏。Pristane诱导24周， IgG和C3在小鼠肾脏出现沉积 (IF Staining)且小鼠肾脏出现炎症浸润和系膜细胞增生(HE Staining)。尿生化分析Pristane诱导24周后，尿液中总蛋白，微量白蛋白，肌酐，尿素氮水平均有不同程度的升高。Case Study: UV诱导的皮肤红斑狼疮(CLE)模型结果输出Back skin and Kidney: H&E stainingBack skin: FACS analysisBack skin and Serum: Cytokine analysis
Application: The model highly simulates the immune microenvironment and histopathological features of human CLE, enabling systematic research from molecular mechanisms to clinical translationLimitation: The current model mainly focuses on skin lesions, and the simulation of multi-organ involvement in systemic lupus erythematosus (SLE) needs further optimizationUV诱导的皮肤组织病理学变化及炎症细胞的浸润
Skin exposure to UV induced epidermal and dermal hyperplasia.
Subacute UV irradiation induced a robust tissue response with a large influx of CD45+ cells; this response peaked at 24h.
Following UV irradiation, the proportion of T cells decreased to ~8% at 24h before returning to No-UV irradiation levels at 72h.
Monocytes, macrophages and neutrophils in skin increased after UV irradiation, and the infiltration reached peak at 24h.
UV诱导的血清和皮肤组织中细胞因子的水平变化Skin sample
Serum sample
UV irradiation induced production of proinflammatory cytokines and type I IFN; this response peaked at 3 hours.
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炎症性肠病 (IBD)Case Study: DSS诱导的小鼠溃疡性结肠炎(UC)模型实验动物: C57BL/6, 7 weeks, female造模试剂: 硫酸葡聚糖钠盐 DSS造模方法:连续7天自由饮用DSS水结果输出临床观察: 疾病活动指数DAI评分, 体重组织病理学: H&E分子水平检测: 细胞因子分析(CBA, MSD, ELISA)临床、组织学评估及细胞因子分析
Case Study: TNBS诱导的小鼠克罗恩病(CD)模型实验动物: C57BL/6, 7 weeks, female造模试剂: 三硝基苯磺酸 TNBS造模方法:致敏: day 0 on back, 激发: day7, intrarectal结果输出临床观察: 疾病活动指数DAI评分, 体重组织病理学: H&E分子水平检测: 细胞因子分析(CBA, MSD, ELISA)临床和组织学评估
Case Study: TNBS诱导的大鼠克罗恩病(CD)模型实验动物: SD rats, male造模试剂: 三硝基苯磺酸 TNBS造模方法:day0, intrarectal结果输出临床观察: 疾病活动指数DAI评分, 体重组织病理学: H&E分子水平检测: 细胞因子分析(CBA, MSD, ELISA)临床和组织学评估
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特应性皮炎 (AD)Case Study: 恶唑酮(OXA)诱导的特应性皮炎(AD)模型实验动物: Balb/c, 6-8 weeks, female造模试剂: 恶唑酮 OXA造模方法: 致敏: day 0 on back; 激发: day7-21 on right ear, 7 times结果输出临床观察: 耳朵厚度, 体重组织病理学: H&E细胞, 分子水平检测: 流式分析FACS, Total IgE, 细胞因子分析(CBA, MSD, ELISA)临床、组织学评估及Total IgE分析
Case Study: MC903诱导的特应性皮炎(AD)模型实验动物: C57BL/6, 7-8 weeks, female造模试剂: MC903造模方法: Topically, on ear, 8 times结果输出临床观察: 耳朵厚度, 体重组织病理学: H&E细胞, 分子水平检测: 流式分析FACS, Total IgE, 细胞因子分析(CBA, MSD, ELISA)临床、组织学评估及Total IgE分析
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银屑病 PsoriasisCase Study: 咪喹莫特(IMQ)诱导的银屑病模型实验动物: C57BL/6, 7 weeks, female造模试剂: 咪喹莫特乳膏 IMQ造模方法: 连续6天背部涂抹IMQ结果输出临床观察： PASI评分, 背部皮肤厚度, 体重组织病理学: H&E细胞, 分子水平检测： 流式分析FACS, 细胞因子分析(CBA, MSD, ELISA)临床、组织学评估及细胞因子分析
Case Study: IL-23诱导的银屑病模型实验动物: C57BL/6, 7 weeks, female造模试剂: Recombinant mouse IL-23 and human IL-23造模方法: Intradermal injection, ear, 4 times结果输出临床观察： 耳朵厚度, 体重组织病理学: H&E细胞, 分子水平检测： 流式分析FACS, 细胞因子分析(CBA, MSD, ELISA)临床和组织学评估
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哮喘 AsthmaCase Study: OVA诱导的哮喘模型实验动物: Balb/c, 6-8 weeks, female造模试剂: Ovalbumin (OVA) + Al(OH)3造模方法: 致敏: i.p., on day 0, 7, 14 ; 激发: Intranasal, on day 21-26结果输出临床观察：体重，小鼠健康状况组织病理学：H&E, PAS, Masson染色细胞, 分子水平检测：流式分析FACS, Total IgE, OVA specific IgE, 细胞因子分析(CBA, MSD, ELISA)临床、组织学评估及细胞、分子水平分析
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肝纤维化 Liver FibrosisCase Study: CCl4诱导的肝纤维化模型实验动物:C57BL/6, 6-8 weeks, male造模试剂:CCl4/Olive oil造模方法:BIW, i.p., 8 weeks结果输出临床观察：体重，小鼠健康状况组织病理学：H&E, 天狼星红染色, IHC(α-SMA)血生化检测：ALT, AST, TBIL, ALB, ALP分子水平检测：细胞因子分析(CBA, MSD, ELISA)组织病理学和血生化分析
Case Study: TAA诱导的肝纤维化模型实验动物: C57BL/6, 7-8 weeks, male造模试剂: 硫代乙酰胺 TAA造模方法: TIW, i.p., 8 weeks结果输出临床观察：体重，小鼠健康状况组织病理学：H&E, 天狼星红染色, IHC(α-SMA)血生化检测：ALT, AST, TBIL, ALB, ALP分子水平检测：细胞因子分析(CBA, MSD, ELISA)组织病理学和血生化分析
